Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
Total Phosphatidic Acid Assay Kit (Fluorometric) (MET-5019)
Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
額外資訊
MET-5019
Total Phosphatidic Acid Assay Kit (Fluorometric)
100 assays
Fluorometric
Detection sensitivity of approximately 5 uM phosphatidic acid
Phosphatidic Acid standard curve included
Dry Ice
Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Kit
Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
Detection sensitivity of approximately 5 uM phosphatidic acid
Phosphatidic Acid standard curve included
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