Robustly expand reset naïve human embryonic stem (ES) and induced pluripotent stem (iPS) cells using a defined cell culture medium.
Reset naïve human pluripotent stem cells (hPSCs) cultured in this medium demonstrate uniformly domed, phase-bright colonies with refractive edges and maintain gene expression profiles associated with reset naïve hPSCs (Guo et al. Stem Cell Reports, 2016; Guo et al. Development, 2017; Takashima et al. Cell, 2014). Reset naïve hPSCs cultured in this medium are capable of tri-lineage differentiation into all three somatic lineages following re-priming.
Based on the t2iL + Gö formulation by Austin Smith and developed under license from the Cambridge Stem Cell Institute at the University of Cambridge, UK, this medium is compatible with reset naïve human ES and iPS cells derived using NaïveCult™ Induction Kit (Catalog #05580), transgene-induced reset human pluripotent stem cells (hPSCs) (Takashima et al. 2014), or embryo-derived reset hPSCs (Guo et al. 2016).