Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve.
Soluble Collagen Assay Kit (MET-5016)
Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve.
額外資訊
MET-5016
Soluble Collagen Assay Kit
96 assays
colorimetric
Detection sensitivity of approximately 15 ug/ml collagen
Collagen standard curve included
Room Temp
Upon receipt, store the Sirius Red Reagent at room temperature and store the rest of the kit at 4ºC.
Kit
Detects soluble collagen in cell and tissue lysates
Detection sensitivity of approximately 15 ug/ml collagen
Collagen standard curve included
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