The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.
Radius™ 48-Well Cell Migration Assay (CBA-5037)
The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.
額外資訊
CBA-5037
Radius™ 48-Well Cell Migration Assay
Cell Based Assays
Kit
48 assays
Room Temp
Upon receipt, aliquot and store the Radius™ Gel Removal Solution and DAPI Fluorescence Stain at -20ºC (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4ºC. Store all
Light Microscopy
Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
Allows qualitative, quantitative, endpoint or real-time analysis
Adaptable to liquid handling equipment and HCS instrumentation
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