Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
Platinum-E Retroviral Packaging Cell Line, Ecotropic (RV-101)
Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
額外資訊
RV-101
Platinum-E Retroviral Packaging Cell Line, Ecotropic
Cell LIne
Viral Expression->Retroviral Expression->Retroviral Expression and Packaging->Platinum Retroviral Packaging Cell Lines
Viral Expression
1 vial
Dry Ice
For best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
Human cells transduced with retroviral genes
FOR SALE TO ACADEMIC, GOVT AND NON-PROFIT LABS ONLY
Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection
Longer stability: up to 4 months in the presence of drug selection
Produces ecotropic retrovirus, which can only readily infect mouse or rat cells
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