First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.
OxiSelect™ Nε-(carboxyethyl) lysine (CEL) Competitive ELISA Kit (STA-813)
First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.
額外資訊
STA-813
OxiSelect™ Nε-(carboxyethyl) lysine (CEL) Competitive ELISA Kit
96 assays
colorimetric
Perform up to 96 assays, including standard curve and unknown protein samples
Will not cross react with CML protein adducts
2 parts: 1 RT / 1 Blue Ice
Upon receipt, aliquot and store the CEL-BSA Standard, CEL Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
Kit
Provides rapid detection and quantitation of CEL protein adducts
Perform up to 96 assays, including standard curve and unknown protein samples
Will not cross react with CML protein adducts
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