The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
OxiSelect™ Hydrogen Peroxide / Peroxidase Assay Kit (Fluorometric) (STA-344)
The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
額外資訊
STA-344
OxiSelect™ Hydrogen Peroxide / Peroxidase Assay Kit (Fluorometric)
500 assays
Fluorometric
Suitable for use with cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine, or other biological fluids
Simple assay protocol provides results in 30-90 minutes, depending on sample type
Room Temp
Upon receipt, aliquot and store the ADHP probe, HRP at -20ºC. Avoid multiple freeze/thaw cycles. Store the remaining kit components at 4ºC. Protect ADHP from light.
Kit
Detect hydrogen peroxide concentrations as low as 50 nM
Suitable for use with cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine, or other biological fluids
Simple assay protocol provides results in 30-90 minutes, depending on sample type
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