BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
OxiSelect™ BPDE Protein Adduct ELISA Kit (STA-301)
BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC. The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.
額外資訊
STA-301
OxiSelect™ BPDE Protein Adduct ELISA Kit
96 assays
colorimetric
BPDE-BSA standard included
Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates
2 parts: 1 RT / 1 Blue Ice
Upon receipt, aliquot and store the Reduced BSA and BPDE-BSA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.
Kit
For detection and quantitation of benzo(a)pyrene diol epoxide (BPDE) protein adducts
BPDE-BSA standard included
Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates
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