Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.
Nuclear/Cytosolic Fractionation Kit (AKR-171)
Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.
額外資訊
AKR-171
Nuclear/Cytosolic Fractionation Kit
20 Preps
N/A
Extraction in less than 2 hours
Each prep can accommodate up to 5 x 106cells
Blue Ice
Upon receipt, aliquot and store Dithiothreitol and Protease Inhibitor Cocktail at -20ºC and avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Kit
Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells
Extraction in less than 2 hours
Each prep can accommodate up to 5 x 106cells
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