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The antibody produces a strong postive labeling of LHRH at dilutions of 1/200-1/400 using indirect immunofluorescence and at dilutions of 1/2,000 – 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.
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| Tested Application | Immunohistochemistry, Immunocytochemistry, Immunofluoresence |
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| PubMed ID | 2341616, 2197079, 2073580, 2066081, 1765781, 1740548, 1600625, 1505732, 1430339, 26441494 |
| Image Description | Immunohistochemical image of of staining for LNRH/GnRH in the rat median eminence. |
