Our Glycogen Assay Kit (Colorimetric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit’s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve.
Glycogen Assay Kit (Fluorometric) (MET-5023)
Our Glycogen Assay Kit (Colorimetric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit’s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve.
額外資訊
MET-5023
Glycogen Assay Kit (Fluorometric)
100 assays
Fluorometric
Detection sensitivity of 3.75uM
Glycogen standard included
Blue Ice
Upon receipt, store the Glycogen Standard, Fluorometric Probe, HRP, Amyloglucosidase, and Glucose Oxidase at -20ºC. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid
Kit
Suitable for use with serum, plasma, urine, lysates, and cell culture supernatants
Detection sensitivity of 3.75uM
Glycogen standard included
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