Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
DAG (Diacylglycerol) Assay Kit (MET-5028)
Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
額外資訊
MET-5028
DAG (Diacylglycerol) Assay Kit
100 assays
Fluorometric
Detection sensitivity of 4uM DAG
DAG standard included
Dry Ice
Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
Kit
Suitable for use with cell lysates
Detection sensitivity of 4uM DAG
DAG standard included
NOTE: Each sample replicate requires 2 assays for proper data calculation.
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