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The CGRP Antibody was raised to rat alpha-CGRP coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven fluorescein staining at a 1/100-1/200 dilution and a strong Biotin-Streptadvidin/HRP staining at a 1/2000-1/4000 dilution in rat amygdala, and spinal cord. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α -CGRP and partially eliminated by pre-adsorption with rat Ÿ -CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y.
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| Reactivity | Bullfrog, Cat, Dog, Hamster, Human, Mouse, Mudpuppy, Ovine (Sheep), Pig, Platypus, Ram (Sheep), Rat |
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| Tested Application | Immunohistochemistry, Immunocytochemistry, Immunofluoresence |
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| PubMed ID | 3711894, 2794128, 1981998, 1699158, 1587996, 1479068, 1385495, 1384974, 24732837, 25326085 |
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