ACTH (Adrenocorticotropic Hormone) Antibody (20070)

The ACTH Antibody was raised to ACTH (1-39) purified from porcine pituitary. The antibody produces a maximum fluorescein staining at a 1/100 – 1/200 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/500 – 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series are 1/100-1/200 in PBS/0.3% Triton X-100 – FITC Technique and 1/500-1/1000 in PBS/0.3% Triton X-100 – Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of ACTH.