FMRF-amide (Cardio-excitatory Peptide) Antibody (20091)

The FMRF-Amide antiserum was quality control tested using standard histochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 – 1/800 in PBS/0.3% Triton X-100 -Cy3 Technique and 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of FMRF-Amide.