<strong>C57BL/6 mouse dermal microvascular endothelial cells</strong> are isolated from tissue of pathogen-free laboratory mice. <strong>C57BL/6 mouse dermal microvascular endothelial cells</strong> are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.