NeoScientific

Custom Recombinant Protein Services from NeoScientific

Step 1: Gene Synthesis & Cloning
Amplification/isolation of the gene of interest
Sub-cloning into an expression vector(s)
Verification of gene sequence

Step 2: Pilot Expression Testing
Transformation of constructs into host cells
Small-scale expression of the target protein
Evaluation of optimal protein expression conditions

Step 3: Large-Scale Protein Expression & Purification
Large-scale growth of over-expressing cells
Protein purification by affinity, ion exchange, gel filtration, or hydrophobic interaction
SDS-PAGE or Western blot analysis of the purified protein
Determination of protein concentration.