{"id":224497,"date":"2022-03-18T02:20:40","date_gmt":"2022-03-17T18:20:40","guid":{"rendered":"https:\/\/www.interlabbiotech.com\/wp\/product\/rapad-mirna-adenoviral-expression-system-vpk-253"},"modified":"2022-03-18T02:20:47","modified_gmt":"2022-03-17T18:20:47","slug":"rapad-mirna-adenoviral-expression-system-vpk-253","status":"publish","type":"product","link":"https:\/\/www.interlabbiotech.com\/wp\/product\/rapad-mirna-adenoviral-expression-system-vpk-253","title":{"rendered":"RAPAd\u00ae miRNA Adenoviral Expression System (VPK-253)"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"<p>Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd\u00ae Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd\u00ae system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5&#8242; ITR, packaging signal and E1 sequences have been removed.<\/p>\n","protected":false},"featured_media":224071,"template":"","meta":[],"product_brand":[],"product_cat":[370180,371576],"product_tag":[],"class_list":{"0":"post-224497","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-cell-biolabs","7":"product_cat-expression-system-vectors","8":"pa_comment-cloning-capacity-of-shuttle-vector-5-2-kb","9":"pa_note-generate-high-titer-virus-in-about-2-3-weeks-compared-to-2-3-months-with-other-systems","10":"pa_product-line-viral-expression","11":"pa_product-name-rapad-mirna-adenoviral-expression-system","12":"pa_product-no-vpk-253","13":"pa_product-type-kit","14":"pa_remark-engineered-to-produce-100-to-10000-fold-fewer-wild-type-plaques-than-most-other-expression-systems","15":"pa_sample-type-generate-high-titer-virus-in-about-2-3-weeks-compared-to-2-3-months-with-other-systems","16":"pa_sensitivity-limit-cloning-capacity-of-shuttle-vector-5-2-kb","17":"pa_shipping-temp-blue-ice","18":"pa_size-1-kit","19":"pa_storage-temp-20oc","21":"first","22":"instock","23":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product\/224497","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/media\/224071"}],"wp:attachment":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/media?parent=224497"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product_brand?post=224497"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product_cat?post=224497"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product_tag?post=224497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}