{"id":191323,"date":"2022-03-09T17:13:19","date_gmt":"2022-03-09T09:13:19","guid":{"rendered":"https:\/\/www.interlabbiotech.com\/wp\/product\/balb-c-mouse-cardiac-fibroblasts-abc-tc3064"},"modified":"2022-03-09T17:13:20","modified_gmt":"2022-03-09T09:13:20","slug":"balb-c-mouse-cardiac-fibroblasts","status":"publish","type":"product","link":"https:\/\/www.interlabbiotech.com\/wp\/product\/balb-c-mouse-cardiac-fibroblasts","title":{"rendered":"BALB\/c Mouse Cardiac Fibroblasts (ABC-TC3064)"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"<p>BALB\/c Mouse Primary Cardiac Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Cardiac Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1&#215;10^6 cells per ml and is delivered frozen.<br \/>\nMouse Primary Cardiac Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1\/S100A4 by immunofluorescence staining. Cells can be expanded for 2-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.<br \/>\nStandard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.<\/p>\n","protected":false},"featured_media":191157,"template":"","meta":[],"product_brand":[],"product_cat":[312445,315260],"product_tag":[],"class_list":{"0":"post-191323","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-accegen","7":"product_cat-primary-cells","8":"pa_category-2-primary-cells","9":"pa_product-name-balb-c-mouse-cardiac-fibroblasts","10":"pa_product-no-abc-tc3064","11":"pa_shipping-info-dry-ice","12":"pa_size-1-vial","13":"pa_species-balb-c-mouse","14":"pa_storage-liquid-nitrogen","15":"pa_supplier-company-accegen-biotechnology","17":"first","18":"instock","19":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product\/191323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/media\/191157"}],"wp:attachment":[{"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/media?parent=191323"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product_brand?post=191323"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product_cat?post=191323"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.interlabbiotech.com\/wp\/wp-json\/wp\/v2\/product_tag?post=191323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}