{"version":"1.0","provider_name":"\u5353\u6607\u6709\u9650\u516c\u53f8-\u6700\u5c08\u696d\u79d1\u5b78\u7814\u7a76\u4f9b\u61c9\u5546","provider_url":"https:\/\/www.interlabbiotech.com\/wp","author_name":"admin","author_url":"https:\/\/www.interlabbiotech.com\/wp\/author\/diego","title":"BKS DB Mouse Vein Endothelial Cells","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"65xQIqz4zh\"><a href=\"https:\/\/www.interlabbiotech.com\/wp\/product\/bks-db-mouse-vein-endothelial-cells\">BKS DB Mouse Vein Endothelial Cells (ABC-TC5499)<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/www.interlabbiotech.com\/wp\/product\/bks-db-mouse-vein-endothelial-cells\/embed#?secret=65xQIqz4zh\" width=\"600\" height=\"338\" title=\"BKS DB Mouse Vein Endothelial Cells (ABC-TC5499) &#8212; \u5353\u6607\u6709\u9650\u516c\u53f8-\u6700\u5c08\u696d\u79d1\u5b78\u7814\u7a76\u4f9b\u61c9\u5546\" data-secret=\"65xQIqz4zh\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script type=\"text\/javascript\">\n\/* <![CDATA[ *\/\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/* ]]> *\/\n<\/script>\n","thumbnail_url":"https:\/\/www.interlabbiotech.com\/wp\/wp-content\/uploads\/2022\/03\/Accegen-Cell-image-web.jpg","thumbnail_width":600,"thumbnail_height":370,"description":"BKS db Mouse Vein Endothelial Cells are isolated from the vein of Mice homozygous for the diabetes spontaneous mutation (Lepr\/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5\u00d7106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody."}